Help Center
Frequently Asked Questions
ORDERING, SHIPPING, AND STORAGE QUESTIONS
Which countries do you ship to?
We ship to all countries!
If I order today, when will I receive my order?
Most orders will be shipped in two business days. Special orders such as Elisa Kit will be shipped in five business days. You will be notified by email if longer than expected.
How are the kits shipped?
We use FedEx and UPS to deliver your order quickly and reliably.
What do I do when I receive my kit?
First, you need to check your packing slip to see that you received everything you ordered. If you find any errors, please contact us by email within 2 business days. Then, check the temperature conditions for each box and store appropriately.
At what temperature should the contents of the kit be stored?
Short term (up to 6 months) store at 2-8°C. Long term, aliquot and store at 20°C. Avoid cycles of freezing and thawing.
I left my reagents at room temperature for the weekend, what do I do?
Antibodies are safe to use within 3 months. Antigens are more sensitive to temperature. Replace your order for best results.
How long can the GEArray kits be stored?
When properly stored, the kits are guaranteed for 12 months from the date of shipping.
GENERAL QUESTIONS RELATED TO ELISA
How do I design a monoclonal antibody or Mab-based sandwich ELISA?
You need to find a pair of monoclonal antibodies with different subclass (IgG1, IgG2a, IgG2b, IgG3, or IgM). They can also differ by the light chains, i.e., one Ab with kappa chain and the other with lambda chain. Then, test them one as capture Ab and the other detection, and vice versa. Choose the combination that yields better signals.
How do I determine the antibody concentration in ascites or tissue culture supernatant?
Use a purified immunogloblin of the same subclass/subtype as a calibration standard (see our General Reagent section), run a generic version of ELISA or F-ELISA of your choice or our kits (XXXXX). The concentration in the ascites or tissue culture supernatant can be derived from the standard curve for the purified immunogloblin.
How do I lower the background in the F-ELISA?
The wash step after the incubation of enzyme conjugates is very critical for reducing the background. The suggested practices include tap dry the plates to remove any residual liquid and double the number of wash cycles for this wash step compare to other wash steps, such as wash twice with 3 washes in each wash program and tap dry the plates in between.
When serum samples are tested, how do I lower the background?
The following two methods in the step for serum sample dilution and/or incubation can be used to reduce the background: 1) Higher salt (NaCl, citrate buffers preferred); and/or 2)Inclusion of certain surfactants (such as polysorbate-20, polysorbate-80, or Triton X-100).
How is the sensitivity of F-ELISA compared to the OD version?
F-ELISA is generally 5-10 fold more sensitive than ELISAs using TMB or OPD as substrates.