ELISA Substrates

ViroLabs, Inc. is your ‘one-stop’ and final supplier if you are searching ELISA substrates for your assay development. It offers a full spectrum of all the substrates for commonly used horseradish peroxidase (HRP) and alkaline phosphatase (AP) in most ELISA assays. Our scientists have developed one-component, ready-to-use substrates for all these immobilized enzymes and these high sensitivity, low background substrates have assisted numerous scientists in their assay development and routine ELISA assay performance. Fluorescence-based ELISA (F-ELISA) with 4-MUP (4-methylumbelliferyl phosphate) for AP and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) for HRP are generally 5-10 folds more sensitive than the corresponding colorimetric assay. Whether you prefer the absorbance-based or fluorescence–based ELISA assay, our one component, ready-to-use ELISA substrates will not disappoint you. Our ready-to-use TMB (3,3’,5,5’-tetramethylbenzidine) solution has been widely adopted by many research scientists in immunology, virology and epidemiology areas.

Catalog Number	Unit/Description	Price
TMB-E-100	Size: 100 mL SuperBlu-ELISA, one component, ready-to-use TMB solution for both quantitative and qualitative ELISA	$163.00
TMB-LB-100	Size: 100 mL SuperBlu-Low Background, one component, ready-to-use TMB solution with low background and stable endpoint	$161.00
TMB-T-100	Size: 100 mL SuperBlu-Turbo, one component, ready-to-use TMB solution for qualitative ELISA with ultra sensitivity	$163.00
XPHOS-100	Size: 100 mL SuperPhos, 4-MUP Fluorescence ELISA Solution for AP Conjugates Optimized one component, ready-to-use formulation for the fluorescence detection of alkaline phosphatase (AP) conjugates in ELISA assays.	$163.00
TRED-80	Size: 80 mL SuperRed, one component, ready-to-use ADHP solution for fluorescence ELISA with HRP conjugates. Optimized for quantitative analysis, where titer, antigen concentration, ED50 or IC50 values are being measured.	$183.00
TRED-LB-80	Size: 80 mL SuperRed, one component, low background, ready-to-use ADHP solution for fluorescence ELISA with HRP conjugates. Optimized for the cut-off assay, where the signal-to-background (S/B) ratio needs to be maximized. The specialized formulation has low background, which enables overnight incubation to maximize the difference between the low antigen end of the curve from the blank (or negative control) in the assay.	$195.00
XYELO-100	Size: 100 mL SuperYelo, pNPP absorbance AP substrate detection system	$161.00